Stoddard ST, Morrison AC, Vazquez-Prokopec GM, Paz Soldan V, Kochel TJ, Kitron U, Elder JP, Scott TW, 2009. (= 2) of individuals. Earlier studies show establishment of potential vectors in this area. These evidences support the hypothesis that DF can be a health concern in Southeast Iran with potential future outbreaks. Intro Dengue fever (DF) and dengue hemorrhagic fever (DHF) are two of the most widely spread mosquito-borne disease in Southeast Asia, western Pacific region, and the United States. The disease is definitely caused by dengue disease (DENV), a Flaviviridae with four closely related serotypes (DEN-1, DEN-2, DEN-3, and DENmosquitos. Probably one of the most possible scenarios in this region Epalrestat is the chance of misdiagnosing DF with Epalrestat Crimean Congo hemorrhagic fever (CCHF). CCHF is currently endemic and known in this area. Therefore, the present study was designed to investigate possibility of DF in individuals clinically suspected of having viral hemorrhagic fever but tested bad for CCHF in Sistan and Baluchestan province of Iran. METHODS Sample collection. The study protocol was authorized by Institutional Ethics Committee (Authorization No. IR.ZAUMS.REC.1393.7002) at Zahedan University or college of Medical Sciences. Serum specimens were collected from suspected individuals admitted to Boo-Ali Hospital in Zahedan within the 1st 3 days of admission (April 2013 to August 2015). Suspected instances were interviewed and examined by infectious diseases physicians. Patients showing with compatible symptoms (fever, myalgia, arthralgia, headache, rash, or bleeding) and tested bad for CCHF (PCR, Epalrestat immunoglobulin M [IgM], and immunoglobulin G [IgG]) were recruited to the study. In addition, seven samples were also sent to the hospital from your rural part of Baluchestan area (Saravan) from suspected individuals HLA-G presented with related presentations within the 1st 3 days of symptoms onset. Samples were tested for anti-dengue disease IgM, IgG, and nonstructural protein 1 (NS1) antigen. Test overall performance. Disease isolation, PCR, or antigen detection can be used to diagnose DF during acute febrile illness. Regrettably, PCR and viral tradition were not available at the time of study, and we decided to use combination of serology and antigen detection. IgM, IgG antibodies, and NS1 antigen were tested using commercial enzyme-linked immunosorbent assay (ELISA) Epalrestat packages offered from Euroimmune AG, Luebeck, Germany (research no: EI 266b-9601 M, EI 266b-9601 G, and EQ 266a-9601-1, respectively). The optical denseness (OD) of each sample was examined in the wavelength of 450 nm and the research wavelength was 620C650 nm. The OD of samples was compared with the calibrator. Per manufacturers instruction, the result was interpreted bad if the percentage of the sample reading to caliber was 0.8, borderline if the percentage was 0.8 and 1.1, and positive if the percentage was 1.1. RESULTS In this study, a total of 60 individuals (36 males and 24 females) met inclusion criteria. Overall, 13 individuals (7 males and 6 females; imply age Epalrestat of 30 years) experienced evidence of past or recent exposure to DENV (Table 1). Five individuals had positive test results in favor of acute infection. None of patients experienced travel history outside Iran. Table 1 Result of dengue disease test studies in 13 Iranian individuals showing with fever, rash, headache, and myalgia in Sistan and Baluchestan, Iran (2013C2015) in southern Iran.17 The varieties is most well-known for transmitting dengue and chikungunya viruses. In another study, was also recognized in the southeast of Iran (2012C2014). This mosquito varieties has been reported like a dengue vector in Karachi, Pakistan.18 These studies support establishment of DENV vectors in this area. This study helps the hypothesis that DENV circulates in patient human population in the southeast of Iran and displays the fact that the risk of DENV outbreaks in this area is greater than what was thought before. These results could be also evidence of small outbreaks which were not large plenty of to attract attention from public health authorities, although creating a national monitoring system to monitor annual number of cases throughout the country would be an ideal response to this report to collect data and set up infrastructures for future research work and outbreak response. Finally, studies for finding additional potential vector varieties, that is, mosquitos with this.